Zhitong Jiangu decoction mitigates osteoarthritis in rabbits via regulation of NF-κB signaling pathway


 Purpose: To investigate the effect of Zhitong Jiangu decoction (ZJD) on osteoarthritis rabbits, and mechanism action involved.
 Methods: Chondrocytes were obtained from knees osteoarthritic rabbits. These chondrocytes randomly assigned to 7 groups: sham, 5, 10 20 % normal serum groups; 5 ZJD, ZJD groups. The gross histopathological features rabbit cartilage examined by microscopy. Each group was treated with a different concentration or drug-containing serum. protective concentrations cells determined. Cell proliferation IL-1 MMP-3 determined using cell counting kit (CCK) 8 enzyme-linked immunosorbent assay (ELISA), respectively. mRNA protein expressions inhibitor nuclear factor-κB kinase α (IKK-α) factor kappa B p65 (NF- κB p65) quantitative reverse transcription polymerase chain reaction (qRT- PCR) immunoblotting, as appropriate.
 Results: Gross examinations showed that successfully established in knee joint. significantly time- concentration-dependently increased groups, relative sham serum-containing However, NF-κB p65, IL-1, IKK-α markedly reduced groups (p < 0.05).
 Conclusion: results indicate mitigates rabbits via regulation signaling pathway. Thus, it can potentially be developed for management osteoarthritis.